Pesticides are chemical compounds used to kill pests, including insects, rodents, fungi and harmful plants (weeds). In addition, they are also used in public health to kill the vectors of diseases such as mosquitoes. Because they may cause potential toxicity to other organisms, including humans, pesticides must be used safely and handled properly1.
At work, exposure to pesticides at home or in the garden may result in exposure to pesticides, for example through contaminated food. The WHO reviews the evidence and sets internationally recognized maximum residue limits to protect people from potential health hazards caused by pesticides. 2
Reversed-phase high performance liquid chromatography (HPLC) is commonly used to estimate the concentration of active ingredients in pesticides. However, this type of chromatography requires the use of toxic solvents, and is time-consuming and well-trained operators, resulting in high costs for routine analysis. Using visible near infrared spectroscopy (Vis-NIRS) instead of HPLC can save time and money.
To test the effectiveness of using Vis-NIRS instead of HPLC, 24-37 pesticide samples with known effective compound concentrations were prepared: abamectin EC, amimectin EC, cyfluthrin EC, cypermethrin, and glyphosate. Assess the correlation between changes. Spectral data and reference values.
NIRS RapidLiquid analyzer is used to obtain the spectrum of its entire wavelength range (400-2500 nm). The sample is put into a disposable glass bottle with a diameter of 4 mm. Vision Air 2.0 Complete software is used for data collection and management as well as quantitative method development. Partial least squares (PLS) regression was performed on each sample analyzed, and internal cross-validation (leave one out) was applied to confirm the performance of the quantitative model derived during the method development.
Figure 1. The NIRS XDS RapidLiquid analyzer is used for spectral data acquisition over the entire range of 400 nm to 2500 nm.
In order to quantify each compound in the pesticide, a model using two factors was established, with a calibration standard error (SEC) of 0.05% and a cross-validation standard error (SECV) of 0.06%. For each effective compound, the R2 values ​​between the provided reference value and the calculated value are 0.9946, 0.9911, 0.9912, 0.0052, and 0.9952, respectively.
Figure 2. Raw data spectra of 18 pesticide samples with abamectin concentrations between 1.8% and 3.8%.
Figure 3. Correlation graph between the abamectin content predicted by Vis-NIRS and the reference value evaluated by HPLC.
Figure 4. The raw data spectra of 35 pesticide samples, in which the concentration range of amomycin is 1.5-3.5%.
Figure 5. Correlation graph between the amimectin content predicted by Vis-NIRS and the reference value evaluated by HPLC.
Figure 6. Raw data spectra of 24 pesticide samples with cyfluthrin concentrations of 2.3–4.2%.
Figure 7. Correlation graph between the cyfluthrin content predicted by Vis-NIRS and the reference value evaluated by HPLC.
Figure 8. The raw data spectra of 27 pesticide samples with cypermethrin concentration of 4.0-5.8%.
Figure 9. Correlation graph between the cypermethrin content predicted by Vis-NIRS and the reference value evaluated by HPLC.
Figure 10. The raw data spectra of 33 pesticide samples with glyphosate concentration of 21.0-40.5%.
Figure 11. Correlation graph between the glyphosate content predicted by Vis-NIRS and the reference value evaluated by HPLC.
These high correlation values ​​between the reference value and the value calculated using Vis-NIRS indicate that it is a highly reliable and much faster method for pesticide quality control compared to the traditionally used HPLC method. Therefore, Vis-NIRS can be used as an alternative to high-performance liquid chromatography for routine pesticide analysis and can save time and money.
Metrohm (2020, May 16). Quantitative analysis of five effective ingredients in pesticides by visible light near infrared spectroscopy. AZoM. Retrieved from https://www.azom.com/article.aspx?ArticleID=17683 on December 16, 2020.
Metrohm “quantified five active ingredients in pesticides through visible and near infrared spectroscopy.” AZoM. December 16, 2020. .
Metrohm “quantified five active ingredients in pesticides through visible and near infrared spectroscopy.” AZoM. https://www.azom.com/article.aspx?ArticleID=17683. (Accessed on December 16, 2020).
Metrohm Corporation in 2020. Quantitative analysis of five effective ingredients in pesticides was carried out by visible and near infrared spectroscopy. AZoM, viewed on December 16, 2020, https://www.azom.com/article.aspx? ArticleID = 17683.
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