Ko te waikawa abscisic hormone tipu (ABA) he kaiwhakahaere nui mo te urutaunga ahotea abiotic.Ko te mana o te pūmua PP2C kaiawhina penei i te ABI1 ko te pokapū pokapū o te whakawhiti tohu ABA.I raro i nga tikanga paerewa, ka herea te ABI1 ki te kinase protein SnRK2s me te aukati i tana mahi.Ko te ABA e herea ana ki te pūmua kaiawhina PYR1/PYLs e whakataetae ana me SnRK2s ki te aro ki te ABI1, na reira ka tukuna nga SnRK2 me te whakahohe i te whakautu ABA.
Ko te roopu rangahau i arahina e Ahorangi Xie Qi mai i te Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences kua roa e ako ana i te ubiquitination, he tikanga whakarereke i muri i te whakamaoritanga e whakahaere ana i te tohu ABA.Ko ta raatau mahi o mua i whakaatu i te endocytosis o PYL4 i takawaenga e te ubiquitination o te E2-rite te pūmua VPS23, me te ABA e whakatairanga ana i te XBAT35 ki te whakaheke i te VPS23A, na reira ka tuku i te paanga aukati ki te PYL4 receptor ABA.Heoi, ahakoa ko te tohu tohu ABA e uru ana ki nga pūmua E2 motuhake e hiahiatia ana mo te ubiquitination, me pehea te tohu ABA e whakahaere ana i te ubiquitination kaore ano kia tino marama.
I tata nei, i tautuhia e ratou tetahi E2 enzyme UBC27 motuhake, he pai te whakahaere i te matewai me te urupare ABA i roto i nga tipu.Na roto i te tātaritanga IP / MS, i whakatauhia e ratou ko te ABA co-receptor ABI1 me te RING-momo E3 ligase AIRP3 kei te taunekeneke nga pūmua o UBC27.
I kitea e ratou ko te UBC27 te taunekeneke me te ABI1 me te whakatairanga i tana whakahekenga, me te whakahohe i te mahi E3 o AIRP3.Ko AIRP3 te mahi hei E3 ligase o ABI1.
I tua atu, ko te ABI1 te whakamahi i te epistasis o UBC27 me AIRP3, ko te mahi a AIRP3 ko te UBC27-ti'aturi.I tua atu, ko te maimoatanga ABA e akiaki ana i te whakahuatanga o te UBC27, te aukati i te whakahekenga o te UBC27, me te whakarei ake i te taunekeneke i waenga i te UBC27 me te ABI1.
Ko enei hua e whakaatu ana i te matatini E2-E3 hou i roto i te whakahekenga o te ABI1 me te ture nui me te matatini o te tohu ABA e te punaha ubiquitination.
Ko te taitara o te pepa ko "UBC27-AIRP3 ubiquitination complex regulates ABA signing by promoting the degradation of ABI1 in Arabidopsis thaliana."I whakaputaina i runga ipurangi i runga i te PNAS i te Oketopa 19, 2020.
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