The osisi hormone abscisic acid (ABA) bụ ihe dị mkpa na-achịkwa na osisi abiotic nrụgide mmegharị.Njikwa onye na-anata protein PP2C dị ka ABI1 bụ etiti etiti ntụgharị akara ngosi ABA.N'okpuru ọnọdụ ọkọlọtọ, ABI1 na-ejikọta protein kinase SnRK2s ma gbochie ọrụ ya.ABA jikọtara ya na protein nnabata PYR1/PYL na-asọ mpi na SnRK2s n'ịchụso ABI1, si otú a na-ahapụ SnRK2s ma mee ka nzaghachi ABA rụọ ọrụ.
Ndị otu nyocha nke Prọfesọ Xie Qi sitere na Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences na-amụ ogologo oge na-amụ ubiquitination, usoro mgbanwe ntụgharị post-translational nke na-achịkwa akara ngosi ABA.Ọrụ ha gara aga kpughere endocytosis nke PYL4 nke mgbasa ozi sitere na mgbasa nke E2-dị ka protein VPS23, na ABA na-akwalite XBAT35 iji mebie VPS23A, si otú ahụ na-ahapụ mmetụta mgbochi na onye na-anabata ABA PYL4.Agbanyeghị, ma akara ngosi ABA gụnyere protein E2 akọwapụtara nke achọrọ maka ibibi, yana etu akara ngosi ABA si chịkwaa nbibi aghọtabeghị nke ọma.
Na nso nso a, ha chọpụtara kpọmkwem E2 enzyme UBC27, nke na-achịkwa nke ọma nnabata mmiri ozuzo na nzaghachi ABA na osisi.Site na nyocha IP / MS, ha kpebiri na ABA ngalaba-anabata ABI1 na RING-ụdị E3 ligase AIRP3 na-emekọrịta ihe na-edozi protein nke UBC27.
Ha chọpụtara na UBC27 na-emekọrịta ihe na ABI1 ma kwalite mmebi ya, ma mee ka ọrụ E3 rụọ ọrụ AIRP3.AIRP3 na-arụ ọrụ dị ka E3 ligase nke ABI1.
Na mgbakwunye, ABI1 na-arụ ọrụ epistasis nke UBC27 na AIRP3, ebe ọrụ AIRP3 na-adabere na UBC27.Na mgbakwunye, ọgwụgwọ ABA na-eme ka ngosipụta nke UBC27, na-egbochi mmebi nke UBC27, ma na-eme ka mmekọrịta dị n'etiti UBC27 na ABI1 dịkwuo elu.
Nsonaazụ ndị a na-ekpughe mgbagwoju anya E2-E3 ọhụrụ na mmebi nke ABI1 na iwu dị mkpa na mgbagwoju anya nke ABA na-egosi site na usoro ubiquitination.
Aha akwụkwọ a bụ "UBC27-AIRP3 ubiquitination complex na-achịkwa akara ngosi ABA site n'ịkwalite mmebi nke ABI1 na Arabidopsis thaliana."E bipụtara ya na ntanetị na PNAS na Ọktoba 19, 2020.
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